Endocrine Abstracts

The use of more tissue-mimetic 3-dimensional (3D) cell culture models for in vitro drug screening has significantly increased in the past with the expectation to overcome with over-interpretation of drug effects obtained with conventional 2D cultures. In this study, we characterized 3D prostate cancer (PCa) organoids where PCa epithelial cells (LNCaP, DuCaP, LAPC4) were cultured alone or with PCa-associated fibroblasts (CAFs) in 96 well hanging drop plates in order to...

Mitochondria play a vital role in cellular bioenergetics, providing energy via oxidative phosphorylation (OXPHOS) and metabolic intermediates. Aerobic ATP production is orchestrated by a multi-enzyme complex, which uses the electrons gained during oxidation of energy substrates to generate an electrochemical potential across the mitochondrial (mt) membrane that drives ATP synthase. Although there is evidence that mitochondrial function as well as mtDNA mutations might play a r...